2020/12/21 12:03:21
高通量組織研磨儀針對中藥藥材DNA提取的研磨方法
Source：Shanghai Jingxin
實驗目的
通過提取藥材的DNA，進行DNA條形碼分子對照，進而鑒定中藥。實驗原理
通過對照藥材自身獨有的DNA條形碼記錄，可以準確鑒定出中藥材品種。用DNA條形碼分子法鑒定中藥是藥典中一項重要的鑒定方法。
實驗材料和器具
樣品：甘草，陳皮，阿膠飲片
儀器：高通量組織研磨儀（上海凈信，Tissuelyser-24）
耗材：不銹鋼研磨罐，研磨珠（上海凈信）
實驗步驟
需了解具體步驟，請致電廠家
注意事項
1. 研磨罐和研磨珠需充分清洗干凈并烘干備用；若需提取藥材的RNA，則研磨罐和破碎珠要確保滅酶；
2. 每個研磨罐大約可研磨適量大小的藥材量（具體根據樣品本身的緊密度會有變動，一般樣品體積不超過研磨罐容積的三分之二）；
3. 如果放入的樣品沒有脫水或者是水分含量較大的藥草類，需用液氮預冷，具體操作是：把裝好樣品和研磨珠的研磨罐浸入液氮中，直至液氮不沸騰，再迅速將研磨罐放入研磨儀中，設置參數單位振頻，間歇幾十秒后啟動研磨儀，就能得到較好的研磨效果。較堅硬的樣品無需液氮冷凍，如阿膠、鹿茸等。
研磨效果
Previous：高通量組織研磨儀對經濟作物水稻、小麥種子的研磨
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